胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for Caspase 3 (CASP3)

CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

  • 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • SEA626Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測胱天蛋白酶3(CASP3),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數。

實驗原理

將胱天蛋白酶3(CASP3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的胱天蛋白酶3(CASP3)與連接于固相載體上的抗體結合,然后加入生物素化的胱天蛋白酶3(CASP3)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶3(CASP3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

增值服務

檢測試劑盒單組分試劑
ELISA/CLIA專用裂解液
ELISA/CLIA質控品
酶聯免疫試劑盒定制服務
人類疾病動物模型定制服務
血清定制服務
ELISA/CLIA實驗服務
TGFB1活化劑
實時熒光定量PCR實驗服務
體外診斷(IVD)試劑原料——抗原
體外診斷(IVD)試劑原料——抗體
谷胱甘肽—S轉移酶(GSH-ST)試劑盒
還原型谷胱甘肽(GSH)測定試劑盒
谷胱甘肽還原酶活性系數(GRAC)測定試劑盒
血管緊張素轉換酶測定試劑盒
鏈霉親和素

相關產品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPA626Ra02 胱天蛋白酶3(CASP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Ra01 胱天蛋白酶3(CASP3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA626Ra02 胱天蛋白酶3(CASP3)多克隆抗體 WB; IHC; ICC; IP.
PAA626Ra01 胱天蛋白酶3(CASP3)多克隆抗體 WB; IHC; ICC; IP.
LAA626Ra71 胱天蛋白酶3(CASP3)多克隆抗體(生物素標記) WB; IHC; ICC.
LAA626Ra81 胱天蛋白酶3(CASP3)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
MAA626Ra21 胱天蛋白酶3(CASP3)單克隆抗體 WB; IHC; ICC; IP.
LAA626Ra82 胱天蛋白酶3(CASP3)單克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
HEA626Ra 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA626Ra 胱天蛋白酶3(CASP3)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA626Ra01 胱天蛋白酶3(CASP3)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

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